Subsequently, molecular expression was detected by immunofluorescence, RT‑qPCR and western blotting cell migration was investigated via Transwell assays. Small interfering RNAs were designed to knock down FAK expression. The expression of FAK and EMT‑associated molecules in adenomyosis and control cells were determined by immunohistochemical staining and immunofluorescence at the protein level, and at the mRNA level by reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR). The aim of the present study was to determine whether FAK regulates EMT in adenomyosis and to investigate the potential pathway in this process. Cumulatively, we clarified the critical role played by the IFN-γ/IRF-1/let-7a cluster/EMT pathway in regulating the spread of circulating colorectal cancer cells to the liver, and highlighted the critical role that the hepatitis microenvironment plays in modulating that process.Įpithelial‑mesenchymal transition (EMT) has been associated with the pathogenesis of adenomyosis focal adhesion kinase (FAK) serves an important role in the EMT process. Finally, we showed that modulation of let-7a expression regulated the epithelial-mesenchymal transition in colorectal cancer cell lines, and inhibited their capacity to metastasize in vivo. Out data showed that liver inflammation induces IFN-γ expression, which then downregulates expression of the let-7a cluster through IRF-1 in colorectal cancer cells. We used BALB/c animal models of inflammatory liver diseases to confirm that liver inflammation inhibits CRLM, and then elucidated the molecular mechanisms governing that process. The aim of this study was to confirm the mechanism by which liver inflammation inhibits CRLM. The molecular mechanisms driving this phenomenon remain unclear. However, CRLM occurs infrequently in livers exhibiting signs of hepatitis or cirrhosis, suggesting a role for inflammation in attenuating CRLM. The liver is the most common site of metastasis in patients with colorectal cancer, and colorectal cancer liver metastasis (CRLM) is associated with poor rates of survival.
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